Protocols
Reproducible, version-controlled protocols for wet lab and computational biology. Fork, adapt, and share with your team.
Example protocols
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CRISPR-Cas9 Knockout
Gene knockout using CRISPR-Cas9 ribonucleoprotein delivery in mammalian cells.
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DNA PCR-free WGS
PCR-free whole genome library prep for NovaSeq — high-quality libraries without amplification bias.
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Adaptive Sampling
Real-time selective sequencing on MinION / PromethION for targeted enrichment without library prep.
How protocols work
Version-controlled methods that evolve with your research. Write once, fork everywhere.
Write
Author structured, step-by-step protocols with rich formatting, images, reagent lists, and timing annotations.
Fork
Others fork your protocol to adapt it for their organism, reagents, or equipment — while keeping a link to the original.
Cite
Every protocol version gets a DOI. Cite exact methods in your publications with full provenance tracking.
All Protocols
CRISPR-Cas9 Knockout
Official Advanced
cyanea
Gene knockout using CRISPR-Cas9 ribonucleoprotein delivery in mammalian cells.
DNA PCR-free WGS
Official Intermediate
cyanea
PCR-free whole genome library prep for NovaSeq — high-quality libraries without amplification bias.
Adaptive Sampling
Official Intermediate
cyanea
Real-time selective sequencing on MinION / PromethION for targeted enrichment without library prep.
Chromium scRNA-seq
Official Advanced
cyanea
Single-cell gene expression with Chromium Next GEM — full workflow from cell suspension to sequencing-ready libraries.
TRIzol RNA Extraction
Official Beginner
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Total RNA isolation from cells and tissue samples using TRIzol reagent.
Share your protocols
Publish version-controlled protocols that others can fork, adapt, and cite. Make your methods reproducible and get credit for your work.
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