Skip to main content

Protocols

Reproducible, version-controlled protocols for wet lab and computational biology. Fork, adapt, and share with your team.

Search protocols by name, technique, or category...
3,200+
Protocols
180+
Contributors
12k+
Forks
All Wet Lab Computational Library Prep Extraction Imaging Assay
3,200 protocols
Trending Most forked Recently updated
🧪

broad-institute/rna-extraction-trizol

Popular

Total RNA extraction using TRIzol reagent. Optimized for mammalian tissue samples with high RNA integrity scores.

Wet Lab Extraction Beginner
12 steps ~3 hrs Forked 234x v4.2
🔬

10x-genomics/chromium-scrnaseq-v4

Featured

Single-cell RNA-seq library preparation using the Chromium Next GEM Single Cell 3' Kit v4. Full workflow from cell suspension to sequencing-ready libraries.

Library Prep scRNA-seq Advanced
24 steps ~2 days Forked 189x v4.0
🧬

addgene/gibson-assembly

Gibson Assembly for seamless DNA cloning. One-step, isothermal assembly of multiple overlapping DNA fragments.

Molecular Biology Cloning Intermediate
8 steps ~2 hrs Forked 412x v3.1
💻

sanger-protocols/whole-genome-illumina

Complete whole genome sequencing library preparation for Illumina platforms. Covers DNA extraction through to sequencing QC.

Library Prep WGS Intermediate
18 steps ~1 day Forked 156x v5.0
✂️

openplant/crispr-cas9-plant

New

CRISPR-Cas9 genome editing in plant systems. Covers guide RNA design, Agrobacterium transformation, and screening strategies.

Molecular Biology CRISPR Advanced
15 steps ~5 days Forked 98x v2.3
🧰

neb/end-repair-a-tailing

End repair and A-tailing for NGS library preparation. Compatible with NEBNext Ultra II and similar library prep kits.

Library Prep NGS Beginner
6 steps ~45 min Forked 67x v2.0

Share your protocols

Publish version-controlled protocols that others can fork, adapt, and cite. Make your methods reproducible.

Publish a Protocol